IL-2 Protein (AA 21-169, C160S)

N° du produit ABIN7676700

Cette protéine Recombinant IL-2 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-2 Protein (AA 21-169, C160S) (ABIN7676700)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 21-169, C160S

Séquence: MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF SQSIISTSPQ

Purification: > 90% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 range ≤0.9ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL-2

Sujet: IL2 (Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant mouse IL2 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Poids moléculaire: 17.3 kDa (150aa) confirmed by MALDI-TOF

NCBI Accession: NP_032392

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation